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mouse pkm2 r d systems cat  (R&D Systems)


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    R&D Systems mouse pkm2 r d systems cat
    Figure 5. Nuclear <t>PKM2</t> activity alters the neuronal epigenetic landscape (A) Schematic: phosphorylated PKM2 translocates to the nucleus to interact with transcription factors to regulate gene expression. (B and C) Immunostaining (B) and quantification (C) of p-PKM2(Ser37) (control, n = 8; AD, n = 5). Scale bars, 10 mm. (D and E) Immunostaining (D) and quantification (E) of phosphorylated histone 3 (T11) of MAP2-positive neurons (control, n = 6; AD, n = 5). Scale bars, 10 mm. (F) ATAC-seq profiles around transcriptional start sites of genes regulated by HIF1a, STAT3, and b-catenin (CTNNB1), based on ReMap2020 (control, n = 11; AD, n = 9). (G) HOMER motif enrichment analysis of AD differentially open peaks for HIF1a and STAT3, as previously published (Mertens et al., 2021). (H and I) Differential expression (H) and GO term enrichment (I) of significant genes regulated by HIF1a or STAT3. (B–H) Violin plots: median and quartiles. Significance: unpaired t test, *p < 0.05, **p < 0.01.
    Mouse Pkm2 R D Systems Cat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+pkm2+r+d+systems+cat/pm35987203-230-64-66?v=R%26D+Systems
    Average 90 stars, based on 2 article reviews
    mouse pkm2 r d systems cat - by Bioz Stars, 2026-07
    90/100 stars

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    1) Product Images from "Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer's disease."

    Article Title: Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer's disease.

    Journal: Cell metabolism

    doi: 10.1016/j.cmet.2022.07.014

    Figure 5. Nuclear PKM2 activity alters the neuronal epigenetic landscape (A) Schematic: phosphorylated PKM2 translocates to the nucleus to interact with transcription factors to regulate gene expression. (B and C) Immunostaining (B) and quantification (C) of p-PKM2(Ser37) (control, n = 8; AD, n = 5). Scale bars, 10 mm. (D and E) Immunostaining (D) and quantification (E) of phosphorylated histone 3 (T11) of MAP2-positive neurons (control, n = 6; AD, n = 5). Scale bars, 10 mm. (F) ATAC-seq profiles around transcriptional start sites of genes regulated by HIF1a, STAT3, and b-catenin (CTNNB1), based on ReMap2020 (control, n = 11; AD, n = 9). (G) HOMER motif enrichment analysis of AD differentially open peaks for HIF1a and STAT3, as previously published (Mertens et al., 2021). (H and I) Differential expression (H) and GO term enrichment (I) of significant genes regulated by HIF1a or STAT3. (B–H) Violin plots: median and quartiles. Significance: unpaired t test, *p < 0.05, **p < 0.01.
    Figure Legend Snippet: Figure 5. Nuclear PKM2 activity alters the neuronal epigenetic landscape (A) Schematic: phosphorylated PKM2 translocates to the nucleus to interact with transcription factors to regulate gene expression. (B and C) Immunostaining (B) and quantification (C) of p-PKM2(Ser37) (control, n = 8; AD, n = 5). Scale bars, 10 mm. (D and E) Immunostaining (D) and quantification (E) of phosphorylated histone 3 (T11) of MAP2-positive neurons (control, n = 6; AD, n = 5). Scale bars, 10 mm. (F) ATAC-seq profiles around transcriptional start sites of genes regulated by HIF1a, STAT3, and b-catenin (CTNNB1), based on ReMap2020 (control, n = 11; AD, n = 9). (G) HOMER motif enrichment analysis of AD differentially open peaks for HIF1a and STAT3, as previously published (Mertens et al., 2021). (H and I) Differential expression (H) and GO term enrichment (I) of significant genes regulated by HIF1a or STAT3. (B–H) Violin plots: median and quartiles. Significance: unpaired t test, *p < 0.05, **p < 0.01.

    Techniques Used: Activity Assay, Gene Expression, Immunostaining, Control, Quantitative Proteomics

    Figure 6. A metabolic shift induces AD-like apoptotic competency in human neurons (A and B) Immunostaining (A) and quantification (B) of cleaved caspase-3 over DAPI of bIII-tub-positive control and AD iNs (control, n = 9; AD, n = 7). Scale bars, 50 mm. Green arrows point out Casp3-positive neurons. (C) Schematic: induction of neuronal apoptosis by ABT-737 treatment. (D) Cell death assessed by cleaved caspase 3/bIII-tub-positive cells of control (green) and AD (teal) iNs in response to 0–1 mM ABT-737. (E and F) Immunostaining (E) and quantification (F) of cleaved caspase-3 in bIII-tub neurons after Bcl2 inhibition in control (n = 10) and AD (n = 8) iNs. Scale bars, 50 mm. (G) Pearson correlation analysis of cleaved caspase-3 immunostaining and glycolytic metabolites (UHPLC-MS). (H and I) Control iNs treated with CoDo for 2 days (H) showed increased lactate secretion (vehicle, n = 5; CoDo, n = 5) (I). (J) Quantification of p-PKM2 FI in the nucleus/cytoplasm comparing vehicle-treated (n = 6) and CoDo-treated control iNs (n = 6). (K) Immunostainings of bIII-tub and EGFP fluorescence of EGFP::PKM2 transduced iNs. Dotted lines show cytoplasmic ROI. Scale bars, 100 and 25 mm. (L) Longitudinal EGFP::PKM2 localization in vehicle-treated (n = 3) or CoCl2-treated (n = 4) control iNs. (M and N) Immunostaining (M) and quantification (N) of cleaved caspase-3 positive cells/DAPI before and after ABT-737 (vehicle and CoDo, n = 6; one-way ANOVA, DF: 23, F = 21.34, p < 0.0001, Dunnett’s multiple comparison). Scale bars, 50 mm. Dots represent individual donors throughout the figure. Bars, mean; error bars, SD; significance, unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001.
    Figure Legend Snippet: Figure 6. A metabolic shift induces AD-like apoptotic competency in human neurons (A and B) Immunostaining (A) and quantification (B) of cleaved caspase-3 over DAPI of bIII-tub-positive control and AD iNs (control, n = 9; AD, n = 7). Scale bars, 50 mm. Green arrows point out Casp3-positive neurons. (C) Schematic: induction of neuronal apoptosis by ABT-737 treatment. (D) Cell death assessed by cleaved caspase 3/bIII-tub-positive cells of control (green) and AD (teal) iNs in response to 0–1 mM ABT-737. (E and F) Immunostaining (E) and quantification (F) of cleaved caspase-3 in bIII-tub neurons after Bcl2 inhibition in control (n = 10) and AD (n = 8) iNs. Scale bars, 50 mm. (G) Pearson correlation analysis of cleaved caspase-3 immunostaining and glycolytic metabolites (UHPLC-MS). (H and I) Control iNs treated with CoDo for 2 days (H) showed increased lactate secretion (vehicle, n = 5; CoDo, n = 5) (I). (J) Quantification of p-PKM2 FI in the nucleus/cytoplasm comparing vehicle-treated (n = 6) and CoDo-treated control iNs (n = 6). (K) Immunostainings of bIII-tub and EGFP fluorescence of EGFP::PKM2 transduced iNs. Dotted lines show cytoplasmic ROI. Scale bars, 100 and 25 mm. (L) Longitudinal EGFP::PKM2 localization in vehicle-treated (n = 3) or CoCl2-treated (n = 4) control iNs. (M and N) Immunostaining (M) and quantification (N) of cleaved caspase-3 positive cells/DAPI before and after ABT-737 (vehicle and CoDo, n = 6; one-way ANOVA, DF: 23, F = 21.34, p < 0.0001, Dunnett’s multiple comparison). Scale bars, 50 mm. Dots represent individual donors throughout the figure. Bars, mean; error bars, SD; significance, unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001.

    Techniques Used: Immunostaining, Positive Control, Control, Inhibition, Comparison

    Figure 7. PKM2 inhibition ameliorates PKM2-induced apoptotic competency (A) Schematic: shikonin treatment prevents PKM2 nuclear translocation and increases metabolic enzymatic activity. (B) Longitudinal EGFP::PKM2 localization in vehicle-treated (n = 3), CoCl2-treated (n = 4), and CoCl2+shikonin-treated (n = 4) control iNs.
    Figure Legend Snippet: Figure 7. PKM2 inhibition ameliorates PKM2-induced apoptotic competency (A) Schematic: shikonin treatment prevents PKM2 nuclear translocation and increases metabolic enzymatic activity. (B) Longitudinal EGFP::PKM2 localization in vehicle-treated (n = 3), CoCl2-treated (n = 4), and CoCl2+shikonin-treated (n = 4) control iNs.

    Techniques Used: Inhibition, Translocation Assay, Activity Assay, Control



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    mouse pkm2 r d systems cat  (R&D Systems)
    90
    R&D Systems mouse pkm2 r d systems cat
    Figure 5. Nuclear <t>PKM2</t> activity alters the neuronal epigenetic landscape (A) Schematic: phosphorylated PKM2 translocates to the nucleus to interact with transcription factors to regulate gene expression. (B and C) Immunostaining (B) and quantification (C) of p-PKM2(Ser37) (control, n = 8; AD, n = 5). Scale bars, 10 mm. (D and E) Immunostaining (D) and quantification (E) of phosphorylated histone 3 (T11) of MAP2-positive neurons (control, n = 6; AD, n = 5). Scale bars, 10 mm. (F) ATAC-seq profiles around transcriptional start sites of genes regulated by HIF1a, STAT3, and b-catenin (CTNNB1), based on ReMap2020 (control, n = 11; AD, n = 9). (G) HOMER motif enrichment analysis of AD differentially open peaks for HIF1a and STAT3, as previously published (Mertens et al., 2021). (H and I) Differential expression (H) and GO term enrichment (I) of significant genes regulated by HIF1a or STAT3. (B–H) Violin plots: median and quartiles. Significance: unpaired t test, *p < 0.05, **p < 0.01.
    Mouse Pkm2 R D Systems Cat, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+pkm2+r+d+systems+cat/pm35987203-230-64-66?v=R%26D+Systems
    Average 90 stars, based on 1 article reviews
    mouse pkm2 r d systems cat - by Bioz Stars, 2026-07
    90/100 stars
    		  Buy from Supplier

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    Figure 5. Nuclear PKM2 activity alters the neuronal epigenetic landscape (A) Schematic: phosphorylated PKM2 translocates to the nucleus to interact with transcription factors to regulate gene expression. (B and C) Immunostaining (B) and quantification (C) of p-PKM2(Ser37) (control, n = 8; AD, n = 5). Scale bars, 10 mm. (D and E) Immunostaining (D) and quantification (E) of phosphorylated histone 3 (T11) of MAP2-positive neurons (control, n = 6; AD, n = 5). Scale bars, 10 mm. (F) ATAC-seq profiles around transcriptional start sites of genes regulated by HIF1a, STAT3, and b-catenin (CTNNB1), based on ReMap2020 (control, n = 11; AD, n = 9). (G) HOMER motif enrichment analysis of AD differentially open peaks for HIF1a and STAT3, as previously published (Mertens et al., 2021). (H and I) Differential expression (H) and GO term enrichment (I) of significant genes regulated by HIF1a or STAT3. (B–H) Violin plots: median and quartiles. Significance: unpaired t test, *p < 0.05, **p < 0.01.

    Journal: Cell metabolism

    Article Title: Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer's disease.

    doi: 10.1016/j.cmet.2022.07.014

    Figure Lengend Snippet: Figure 5. Nuclear PKM2 activity alters the neuronal epigenetic landscape (A) Schematic: phosphorylated PKM2 translocates to the nucleus to interact with transcription factors to regulate gene expression. (B and C) Immunostaining (B) and quantification (C) of p-PKM2(Ser37) (control, n = 8; AD, n = 5). Scale bars, 10 mm. (D and E) Immunostaining (D) and quantification (E) of phosphorylated histone 3 (T11) of MAP2-positive neurons (control, n = 6; AD, n = 5). Scale bars, 10 mm. (F) ATAC-seq profiles around transcriptional start sites of genes regulated by HIF1a, STAT3, and b-catenin (CTNNB1), based on ReMap2020 (control, n = 11; AD, n = 9). (G) HOMER motif enrichment analysis of AD differentially open peaks for HIF1a and STAT3, as previously published (Mertens et al., 2021). (H and I) Differential expression (H) and GO term enrichment (I) of significant genes regulated by HIF1a or STAT3. (B–H) Violin plots: median and quartiles. Significance: unpaired t test, *p < 0.05, **p < 0.01.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti Beta-III-tubulin BioLegend Cat#MMS-435P; RRID:AB_2313773 Rabbit anti Beta-III-tubulin BioLegend Cat#802001; RRID:AB_2564645 Mouse anti NeuN EMD Millipore Cat#MAB377; RRID:AB_2298772 Rabbit anti NeuN Cell Signaling Cat#24307T; RRID:AB_2651140 PE-conjugated anti PSA-NCAM Miltenyi Biotec Cat#130-117-394; RRID:AB_2727931 Rabbit anti Synapsin Merck Cat#574778; RRID:AB_565174 Mouse anti PSD-95 (clone K28/42) NeuroMab Cat#75028; RRID:AB_2292909 Chicken anti Map2 Abcam Cat#ab5392; RRID:AB_2138153 Rabbit PKM2 (phospho-Ser37) Sabbiotech Cat#11456 Mouse PKM2 R&D systems Cat#MAB72441 Rabbit Histone 3 (phospho T11) Abcam Cat#ab5168, RRID:AB_304759 Rabbit cleaved caspase 3 (asp175)(5A1E) Cell Signaling Cat#9664; RRID:AB_2070042 Mouse PDH-E1a Santa Cruz Cat#sc-377092; RRID:AB_2716767 Mouse PKM2 ThermoFisher Scientific Cat#TA190266 Alexa Fluor 488-conjugated Donkey Anti Rabbit IgG Thermo Fisher Scientific Cat#A-21206; RRID:AB_2535792 Alexa Fluor 647-conjugated Donkey Anti Rabbit IgG Thermo Fisher Scientific Cat#A-31573; RRID:AB_2536183 Alexa Fluor 488-conjugated Donkey Anti Mouse IgG Thermo Fisher Scientific Cat#A-21202; RRID:AB_141607 Alexa Fluor 647-conjugated Donkey Anti Mouse IgG Thermo Fisher Scientific Cat#A-31571; RRID:AB_162542 Alexa Fluor 647-conjugated Donkey Anti Chicken IgY Millipore Cat#AP194SA6; RRID:AB_2650475 Cy3-conjugated Donkey Anti-Mouse IgG Jackson ImmunoRes.

    Techniques: Activity Assay, Gene Expression, Immunostaining, Control, Quantitative Proteomics

    Figure 6. A metabolic shift induces AD-like apoptotic competency in human neurons (A and B) Immunostaining (A) and quantification (B) of cleaved caspase-3 over DAPI of bIII-tub-positive control and AD iNs (control, n = 9; AD, n = 7). Scale bars, 50 mm. Green arrows point out Casp3-positive neurons. (C) Schematic: induction of neuronal apoptosis by ABT-737 treatment. (D) Cell death assessed by cleaved caspase 3/bIII-tub-positive cells of control (green) and AD (teal) iNs in response to 0–1 mM ABT-737. (E and F) Immunostaining (E) and quantification (F) of cleaved caspase-3 in bIII-tub neurons after Bcl2 inhibition in control (n = 10) and AD (n = 8) iNs. Scale bars, 50 mm. (G) Pearson correlation analysis of cleaved caspase-3 immunostaining and glycolytic metabolites (UHPLC-MS). (H and I) Control iNs treated with CoDo for 2 days (H) showed increased lactate secretion (vehicle, n = 5; CoDo, n = 5) (I). (J) Quantification of p-PKM2 FI in the nucleus/cytoplasm comparing vehicle-treated (n = 6) and CoDo-treated control iNs (n = 6). (K) Immunostainings of bIII-tub and EGFP fluorescence of EGFP::PKM2 transduced iNs. Dotted lines show cytoplasmic ROI. Scale bars, 100 and 25 mm. (L) Longitudinal EGFP::PKM2 localization in vehicle-treated (n = 3) or CoCl2-treated (n = 4) control iNs. (M and N) Immunostaining (M) and quantification (N) of cleaved caspase-3 positive cells/DAPI before and after ABT-737 (vehicle and CoDo, n = 6; one-way ANOVA, DF: 23, F = 21.34, p < 0.0001, Dunnett’s multiple comparison). Scale bars, 50 mm. Dots represent individual donors throughout the figure. Bars, mean; error bars, SD; significance, unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001.

    Journal: Cell metabolism

    Article Title: Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer's disease.

    doi: 10.1016/j.cmet.2022.07.014

    Figure Lengend Snippet: Figure 6. A metabolic shift induces AD-like apoptotic competency in human neurons (A and B) Immunostaining (A) and quantification (B) of cleaved caspase-3 over DAPI of bIII-tub-positive control and AD iNs (control, n = 9; AD, n = 7). Scale bars, 50 mm. Green arrows point out Casp3-positive neurons. (C) Schematic: induction of neuronal apoptosis by ABT-737 treatment. (D) Cell death assessed by cleaved caspase 3/bIII-tub-positive cells of control (green) and AD (teal) iNs in response to 0–1 mM ABT-737. (E and F) Immunostaining (E) and quantification (F) of cleaved caspase-3 in bIII-tub neurons after Bcl2 inhibition in control (n = 10) and AD (n = 8) iNs. Scale bars, 50 mm. (G) Pearson correlation analysis of cleaved caspase-3 immunostaining and glycolytic metabolites (UHPLC-MS). (H and I) Control iNs treated with CoDo for 2 days (H) showed increased lactate secretion (vehicle, n = 5; CoDo, n = 5) (I). (J) Quantification of p-PKM2 FI in the nucleus/cytoplasm comparing vehicle-treated (n = 6) and CoDo-treated control iNs (n = 6). (K) Immunostainings of bIII-tub and EGFP fluorescence of EGFP::PKM2 transduced iNs. Dotted lines show cytoplasmic ROI. Scale bars, 100 and 25 mm. (L) Longitudinal EGFP::PKM2 localization in vehicle-treated (n = 3) or CoCl2-treated (n = 4) control iNs. (M and N) Immunostaining (M) and quantification (N) of cleaved caspase-3 positive cells/DAPI before and after ABT-737 (vehicle and CoDo, n = 6; one-way ANOVA, DF: 23, F = 21.34, p < 0.0001, Dunnett’s multiple comparison). Scale bars, 50 mm. Dots represent individual donors throughout the figure. Bars, mean; error bars, SD; significance, unpaired t test, *p < 0.05, **p < 0.01, ***p < 0.001.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti Beta-III-tubulin BioLegend Cat#MMS-435P; RRID:AB_2313773 Rabbit anti Beta-III-tubulin BioLegend Cat#802001; RRID:AB_2564645 Mouse anti NeuN EMD Millipore Cat#MAB377; RRID:AB_2298772 Rabbit anti NeuN Cell Signaling Cat#24307T; RRID:AB_2651140 PE-conjugated anti PSA-NCAM Miltenyi Biotec Cat#130-117-394; RRID:AB_2727931 Rabbit anti Synapsin Merck Cat#574778; RRID:AB_565174 Mouse anti PSD-95 (clone K28/42) NeuroMab Cat#75028; RRID:AB_2292909 Chicken anti Map2 Abcam Cat#ab5392; RRID:AB_2138153 Rabbit PKM2 (phospho-Ser37) Sabbiotech Cat#11456 Mouse PKM2 R&D systems Cat#MAB72441 Rabbit Histone 3 (phospho T11) Abcam Cat#ab5168, RRID:AB_304759 Rabbit cleaved caspase 3 (asp175)(5A1E) Cell Signaling Cat#9664; RRID:AB_2070042 Mouse PDH-E1a Santa Cruz Cat#sc-377092; RRID:AB_2716767 Mouse PKM2 ThermoFisher Scientific Cat#TA190266 Alexa Fluor 488-conjugated Donkey Anti Rabbit IgG Thermo Fisher Scientific Cat#A-21206; RRID:AB_2535792 Alexa Fluor 647-conjugated Donkey Anti Rabbit IgG Thermo Fisher Scientific Cat#A-31573; RRID:AB_2536183 Alexa Fluor 488-conjugated Donkey Anti Mouse IgG Thermo Fisher Scientific Cat#A-21202; RRID:AB_141607 Alexa Fluor 647-conjugated Donkey Anti Mouse IgG Thermo Fisher Scientific Cat#A-31571; RRID:AB_162542 Alexa Fluor 647-conjugated Donkey Anti Chicken IgY Millipore Cat#AP194SA6; RRID:AB_2650475 Cy3-conjugated Donkey Anti-Mouse IgG Jackson ImmunoRes.

    Techniques: Immunostaining, Positive Control, Control, Inhibition, Comparison

    Figure 7. PKM2 inhibition ameliorates PKM2-induced apoptotic competency (A) Schematic: shikonin treatment prevents PKM2 nuclear translocation and increases metabolic enzymatic activity. (B) Longitudinal EGFP::PKM2 localization in vehicle-treated (n = 3), CoCl2-treated (n = 4), and CoCl2+shikonin-treated (n = 4) control iNs.

    Journal: Cell metabolism

    Article Title: Warburg-like metabolic transformation underlies neuronal degeneration in sporadic Alzheimer's disease.

    doi: 10.1016/j.cmet.2022.07.014

    Figure Lengend Snippet: Figure 7. PKM2 inhibition ameliorates PKM2-induced apoptotic competency (A) Schematic: shikonin treatment prevents PKM2 nuclear translocation and increases metabolic enzymatic activity. (B) Longitudinal EGFP::PKM2 localization in vehicle-treated (n = 3), CoCl2-treated (n = 4), and CoCl2+shikonin-treated (n = 4) control iNs.

    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Antibodies Mouse anti Beta-III-tubulin BioLegend Cat#MMS-435P; RRID:AB_2313773 Rabbit anti Beta-III-tubulin BioLegend Cat#802001; RRID:AB_2564645 Mouse anti NeuN EMD Millipore Cat#MAB377; RRID:AB_2298772 Rabbit anti NeuN Cell Signaling Cat#24307T; RRID:AB_2651140 PE-conjugated anti PSA-NCAM Miltenyi Biotec Cat#130-117-394; RRID:AB_2727931 Rabbit anti Synapsin Merck Cat#574778; RRID:AB_565174 Mouse anti PSD-95 (clone K28/42) NeuroMab Cat#75028; RRID:AB_2292909 Chicken anti Map2 Abcam Cat#ab5392; RRID:AB_2138153 Rabbit PKM2 (phospho-Ser37) Sabbiotech Cat#11456 Mouse PKM2 R&D systems Cat#MAB72441 Rabbit Histone 3 (phospho T11) Abcam Cat#ab5168, RRID:AB_304759 Rabbit cleaved caspase 3 (asp175)(5A1E) Cell Signaling Cat#9664; RRID:AB_2070042 Mouse PDH-E1a Santa Cruz Cat#sc-377092; RRID:AB_2716767 Mouse PKM2 ThermoFisher Scientific Cat#TA190266 Alexa Fluor 488-conjugated Donkey Anti Rabbit IgG Thermo Fisher Scientific Cat#A-21206; RRID:AB_2535792 Alexa Fluor 647-conjugated Donkey Anti Rabbit IgG Thermo Fisher Scientific Cat#A-31573; RRID:AB_2536183 Alexa Fluor 488-conjugated Donkey Anti Mouse IgG Thermo Fisher Scientific Cat#A-21202; RRID:AB_141607 Alexa Fluor 647-conjugated Donkey Anti Mouse IgG Thermo Fisher Scientific Cat#A-31571; RRID:AB_162542 Alexa Fluor 647-conjugated Donkey Anti Chicken IgY Millipore Cat#AP194SA6; RRID:AB_2650475 Cy3-conjugated Donkey Anti-Mouse IgG Jackson ImmunoRes.

    Techniques: Inhibition, Translocation Assay, Activity Assay, Control